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Austria-He-He Azienda Directories
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Azienda News:
- Self-cleaving ribozymes: substrate specificity and synthetic biology . . .
A new type of self-cleaving ribozyme (Hovlinc) has recently emerged through an experimental genome-wide search aimed at uncovering naturally-occurring ribozymes in humans 111,123 While there is limited information about this ribozyme's mechanism of cleavage, RNA structure analysis suggests that the catalytic core consists of two stem-loops
- Engineering a ribozyme cleavage-induced split fluorescent aptamer . . .
Ribozyme engineering and efficient implementation of ribozyme-based genetic switches requires detailed knowledge of individual self-cleavage performances By rational design, we devised fluorescent aptamer-ribozyme RNA architectures that allow for the real-time measurement of ribozyme self-cleavage activity in vitro The engineered nucleic acid
- Chemistry and Biology of Self-Cleaving Ribozymes - Cell Press
Self-cleaving ribozymes were discovered 30 years ago, but their biological distribution and catalytic mechanisms are only beginning to be defined Each ribozyme family is defined by a distinct structure, with unique active sites accelerating the same transesterification reaction across the families Biochemical studies show that general acid-base catalysis is the most common mechanism of self
- RNA-Cleaving NAzymes: The Next Big Thing in Biosensing?
After an initial reported cleavage of a full RNA sequence, the DNAzyme repertoire has been expanded to also include enzymes that cleave DNA sequences harboring RNA bases only in the cleavage site Increasingly, there was a shift in choice of substrates towards the latter, especially for in vitro applications, perhaps owing to the higher
- RNA self-splicing by engineered hairpin ribozyme variants
Cleavage of ribozyme strand 3′HPR proceeds more efficiently (68%), while cleavage of the extended 5′-exon (5′EX OV) is less efficient (41%, Figure 5A and B, lane column F) compared to the scenario shown in Figure 4D, where the 3′-exon (3′EX) and the 3′-intron segment (3′IN) are present instead of 3′HPR (65%, Figure 5A and B
- Ribozyme-activated mRNA trans-ligation enables large gene delivery to . . .
GENE THERAPY Ribozyme-activated mRNA trans-ligation enables large gene delivery to treat muscular dystrophies Sean R Lindley 1,2,3†, Kadiam C Venkata Subbaiah 1,2,3†, Fnu Priyanka , Pornthida Poosala , Yijie Ma 4, Leila Jalinous , Jason A West4, William A Richardson1,2,3, Tamlyn N Thomas 1,2,3, Douglas M Anderson * Ribozymes are small catalytic RNA sequences capable of nucleotide
- Self-cleaving ribozymes conserved in RNA viruses unveil a new . . . - bioRxiv
Small self-cleaving ribozymes are catalytic RNAs originally discovered in viroid-like agents, which are infectious circular RNAs (circRNAs) postulated as relics of a prebiotic RNA world In the last decade, however, small ribozymes have also been detected across the tree of life, from bacterial to human genomes, and more recently, in viral agents with circRNA genomes Here we report the
- Ribozyme-activated mRNA trans-ligation enables large gene delivery to . . .
Ribozymes are small catalytic RNA sequences capable of nucleotide-specific self-cleavage found widespread in nature Ribozyme cleavage generates distinct 2′,3′-phosphate and 5′-hydroxyl termini that resemble substrates for recently characterized RNA repair pathways in cells We report that ribozyme cleavage of two separate mRNAs activated their scarless trans-ligation and translation
- Self-cleaving ribozymes: substrate specificity and synthetic biology . . .
Here, we give an overview of 10 classes of self-cleaving ribozymes and capture what is understood about their substrate specificity and synthetic applications Evolution of these ribozymes in an RNA world might be characterized by the emergence of a new ribozyme family followed by rapid adaptation or diversification for specific substrates
- Chemistry and Biology of Self-Cleaving Ribozymes
Self-cleavage of virusoid RNA is performed by the proposed 55-nucleotide active-site Cell, 50 (1987), pp 9-16 During ribozyme self-cleavage, phosphorane forms the transition state, which can be a true intermediate if it is stabilized through hydrogen bonding of one of the nonbridging phosphates or by a counter ion (e g , a metal ion)
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