|
- Primer designing tool - National Center for Biotechnology Information
Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type Help Entrez query (optional) Help Primer specificity stringency Primer must have at least 1 2 3 4 5 6 total mismatches to unintended targets, including
- National Center for Biotechnology Information
Try "re-search for specific primers" feature on Primer-BLAST result page When only non-specific primers are found, the result page offers the option to re-search for specific primers
- Primer-BLAST Archives - NCBI Insights
To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions It uses these to limit the locations of primers The longest sequence is also used as the representative template sequence in the results
- Neuropathic pain - A clinical primer - PubMed
Neuropathic pain is used both as a mechanistic descriptor and a classification category of pain caused by a lesion or disease of the somatosensory nervous system and encompasses a vast array of possible diagnoses The identification of neuropathic pain and diagnosis of specific syndromes relies on a …
- Nucleotide BLAST: Search nucleotide databases using a nucleotide query
Optimize for Highly similar sequences (megablast) Optimize for More dissimilar sequences (discontiguous megablast) Optimize for Somewhat similar sequences (blastn) Choose a BLAST algorithm Help
- Primer design for RT-PCR - PubMed
Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity
- The Glucocorticoid Taper: A Primer for the Clinicians - PubMed
Affiliations 1 Department of Endocrinology, Fortis Hospital, Mohali, Punjab, India 2 Department of Endocrinology, Sher-i-Kashmir Institute of Medical Sciences
- A rare PRIMER cell state in plant immunity - PubMed
PRIMER cells have non-canonical immune signatures, exemplified by the expression and genome accessibility of a previously uncharacterized transcription factor, GT-3A, which contributes to plant immunity against bacterial pathogens
|
|
|