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- How many clones need to be sequenced from a single forensic or ancient . . .
In this paper, we model ways to help researchers choose the number of clones they need to sequence to obtain the most representative consensus sequences (i e the most abundant sequence in the template mixture) in the presence of template mixture (i e the presence of contaminating DNA molecules from different organisms of the same species or da
- Mammalian cloning: advances and limitations - Nature
Several mammalian species have been cloned by transferring nuclei from various adult somatic cells into enucleated oocytes The cloning procedure is still inefficient, and only one in a hundred
- Isolating, Cloning, and Sequencing DNA - Molecular Biology of the Cell . . .
Selected DNA Segments Can Be Cloned in a Test Tube by a Polymerase Chain Reaction Now that so many genome sequences are available, genes can be cloned directly without the need to construct DNA libraries first A technique called the polymerase chain reaction (PCR) makes this rapid cloning possible PCR allows the DNA from a selected region of
- Cloning - SpringerLink
Cloning is the generation of a genetically identical organism from an existing organism The best known example of cloning is taking plant cuttings, but also in various animals; genetically identical clones can be formed from cells or tissues, for example in some types of flatworms
- Analytical subcloning of a clonal cell line demonstrates cellular . . .
Observations of inherent heterogeneity in cell lines that were originally derived from a single cell, both in this study and throughout the literature,10, 12, 17 calls into question the level of scrutiny one should place around the single cell cloning step when generating a cell line for eventual production of a biotherapeutic
- Cloning Fact Sheet - National Human Genome Research Institute
Researchers routinely use cloning techniques to make copies of genes that they wish to study The procedure consists of inserting a gene from one organism, often referred to as "foreign DNA," into the genetic material of a carrier called a vector
- Regulatory Considerations in Establishing Clonality for Cell lines . . .
For recombinant products, the cell substrate is the transfected cell containing the desired sequences, which has been cloned from a single cell progenitor
- Basic Animal Genetics - Cornell University Department of Animal Science
Cloning is a method of producing two or more genetically identical organisms by asexual reproduction This means that there is only one parent cell, from which all the genetic information will come Thus, the DNA sequence of cloned organisms is exactly the same as that of the parent cell
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