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- pGEM-T Sequence and Map - SnapGene
Parental vector for TA cloning of PCR products The insertion site is flanked by BstZI sites This vector is also known as pGEM®-5Zf (+) Explore Over 2 7k Plasmids: Basic Cloning Vectors | More Plasmid Sets Efficient cleavage requires at least two copies of the NaeI recognition sequence
- pGEM®-T Easy Vector Systems - Promega Corporation
The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments
- Promega pGEM™-T and pGEM™-T Easy Vector Systems - Fisher Sci
The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid
- pGEM-T Easy vector map and sequence
Provide pGEM-T Easy vector plasmid map, full length sequence, antibiotic resistance, size and other information
- pGEM -T and pGEM -T Easy Vector Systems - Perelman School of Medicine . . .
The pGEM®-T and pGEM®-T Easy Vector Systems(a,b) are convenient systems for the cloning of PCR products The vectors are prepared by cutting the pGEM®-5Zf(+) and pGEM®-T Easy Vectors, respectively, with EcoRV and adding a 3 ́ terminal thymidine to both ends
- Cloning with pGEM®-T Vectors: Ligation - Promega Connections
One of the easiest methods for cloning blunt-ended DNA fragments including PCR products is T-vector cloning, such as with pGEM®-T or pGEM®-T Easy Vector Systems This method takes advantage of the “A” overhang added by a PCR enzyme like Taq DNA Polymerase
- pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual - Promega
The pGEM ®-T and pGEM ®-T Easy Vector Systems are convenient systems for the cloning of PCR products The vectors are prepared by cutting the pGEM ® -5Zf(+) and pGEM ® -T Easy Vectors, respectively, with EcoR V and adding a 3´ terminal thymidine to both ends
- pGEM®-3Z Vector - Promega Corporation
The pGEM®-3Z Vector is intended for use as a standard cloning vector, as well as for the highly efficient synthesis of RNA in vitro The vector carries the lac Z α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue white screening
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