Characterization of optimal T Cell Dendritic Cell (DC) Co-Culture . . . To define the optimal culture conditions for human antigen-specific T cell expansion, we conducted an iterative series of pairwise CMV-specific T cell expansions, in which important determinants of the expansion process could be directly compared
[Culture and expansion of CMV-specific T cells derived from . . . - PubMed The phenotypes of T lymphocytes and the secretion levels of tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) before and after amplification were analyzed by polychromatic flow cytometry The titer of serum CMV IgM IgG antibody of the donors was detected by ELISA
Characterization of a Cytomegalovirus-Specific T Lymphocyte . . . - Frontiers We describe a process for generating a single preparation of antiviral T lymphocytes (CD4 + and CD8 +) that is consistently specific for immunodominant and subdominant antigens derived from CMV, a frequent cause of post-transplant morbidity or death
Ex vivo stimulation of cytomegalovirus (CMV)-specific T cells using CMV . . . Cytomegalovirus (CMV) infection is a dangerous complication in immunosuppressed individuals such as allogeneic stem cell transplant patients CMV disease can be prevented by the early post-transplant transfer of donor-derived, CMV-directed, T cells
Generation of high-affinity CMV-specific T cells for adoptive . . . We established a protocol to expand CMV-specific T cells (CMV-T) using peripheral blood mononuclear cells (PBMCs) PBMCs from 16 HLA-A*0201 donors were cultured with a cytokine cocktail comprising IL-2 IL-15 IL-21 along with overlapping peptides from CMV-pp65